stempro adipogenesis differentiation basal medium  (PromoCell)

Journal: Journal of Cellular and Molecular Medicine

Article Title: Bone marrow stromal cells (BMSCs CD45 ‐ /CD44 + /CD73 + /CD90 + ) isolated from osteoporotic mice SAM/P6 as a novel model for osteoporosis investigation

doi: 10.1111/jcmm.16667

Figure Lengend Snippet: Characterization of BMSCs: The inverted light microscope images of BMSC BALB/c and BMSC SAM/P6 . The photographs were captured under 100‐ and 400‐fold magnification. The scale bars are equal 200 µm and 50 µm, respectively (A). The results of metabolic assay (Alamar Blue) determined for both tested population of BMSCs (B). The visualization of cells’ surface markers: CD44, CD73, CD90 and CD45. The microphotographs were taken using confocal microscope under 630× magnification. The scale bar is equal 20 µm (C). The comparative analysis based on staining intensity of surface markers presented as grouped bar graph (D). The images of BMSCs differentiated under osteogenic (E), chondrogenic (F) and adipogenic conditions (G). The photographs of cultures that undergo chondro‐ and osteogenesis were taken by the use of inverted light microscope under 100‐fold magnification, and the scale bar is equal 200 µm. The photographs of cultures that undergo adipogenesis were taken using confocal microscopy in order to visualize cells’ nuclei (DAPI) and lipid droplets (LipidTox). The confocal images were captured under 630‐fold magnification, and the scale bar is equal 40 µm. The stainings intensity measured in differentiated cultures was presented as bar graphs (H, I and J). Significant differences between groups are indicated with asterisks: * P < .05, ** P < .01, *** P < .001. Non‐significant differences are marked as ns

Article Snippet: The adipogenic medium was prepared using StemPro Adipogenesis Differentiation Basal Medium (A10410‐01, Gibco, Life Technologies Corporation, USA) and StemPro Adipogenesis Supplement (A10065‐01, Gibco, Life Technologies Corporation, USA) in the ratio of 10:1, respectively.

Techniques: Light Microscopy, Metabolic Assay, Microscopy, Staining, Confocal Microscopy